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Taken together, these results suggest that LvRac1 is essential in the innate immune response of shrimp to V. alginolyticus infection. Serine protease inhibitors, together with Kunitz-kind protease inhibitors play necessary roles not only in physiological process (i.e. blood clotting and fibrinolysis) but in addition in immune responses. On this study, we characterized a Kunitz-sort protease inhibitor, designated MjKuPI, from kuruma shrimp Marsupenaeus japonicus. On this study, the cDNA of FREP was identified from the kuruma shrimp Marsupenaeus japonicus (MjFREP2). In this examine, a cDNA encoding a RAS GTPase (LvRac1) in the Pacific white shrimp (L. Injection of the bacterium V. alginolyticus into L. vannamei induced hepatopancreatic upregulation of LvRac1 expression. Suppressing LvRac1 additionally promoted the upregulation of Lvp53, LvCu/ZnSOD, and Lvgal following V. alginolyticus injection. The silencing of LvRac1 by double-stranded RNA injection thus elevated the V. alginolyticus problem sensitivity of L. vannamei and weakened its bacterial clearance potential in vivo. Moreover, knocking down LvRac1 in vivo significantly reduced the expression of the L. vannamei p53 and Cu/Zn superoxide dismutase genes (Lvp53 and LvCu/Zn SOD, respectively) whereas growing that of the galectin gene (Lvgal). What are good questions to ask a guy whereas enjoying the question game?